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NFkB-p65(Phospho-Ser536) Antibody

NFkB-p65(Phospho-Ser536) Antibody

  • 商品品牌:AbSci
  • 商品货号:AB11014
  • 货期: 预计到货1-5
货号 规格 价格
AB11014-1 50ul ¥1450 元
AB11014-2 100ul ¥2250 元
AB11014-4 25ul ¥850 元
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Primary Antibody
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
applications WB IHC IF
Application Details

Predicted MW: 65kd

Western blotting: 1:500~1:1000

Immunohistochemistry: 1:50~1:100

Immunofluorescence: 1:100~1:200

Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of NF-kB p65 only when phosphorylated at serine 536.
Target Name NFkB-p65
Alternative Names NFKB3; RELA; TF65; Transcription factor p65; p65
Accession No. Swiss-Prot: Q04206
NCBI Protein: NP_001138610.1
Modification Phospho-Ser536
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 536 (F-S-S(p)-I-A) derived from Human NFkB-p65.
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Background

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.



Doyle S L, et al. (2005) J Biol Chem. 280(25): 23496-23501.

Anwar K N, et al. (2004) J Immunol. 173(11): 6965-6972.

Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.

Baeuerle P A, et al. (1996) Cell 87:13-20.

Haskill S, et al. (1991) Cell 65:1281-1289.

Host Species Rabbit

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