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Histone H3K4me1 Polyclonal Antibody

Histone H3K4me1 Polyclonal Antibody

  • 商品品牌:AbSci
  • 商品货号:ABHW001
  • 货期: 预计到货1-5
货号 规格 价格
ABHW001 100ul ¥2250元
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Primary Antibody
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits and were purified by antigen affinity-chromatography.
applications WB IHC IF
Application Details

WB 1:500 - 1:2000


IHC 1:50 - 1:200

IF 1:50 - 1:200

Species Reactivity Hu Ms Rt
Target Name Histone H3
Alternative Names HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3
Accession No. Gene ID: 8290 Swiss Prot: Q16695
Modification K4me1
SDS-PAGE MW 15kDa
Immunogen Type Peptide
Immunogen Description A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
Concentration 1.0mg/ml
Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
StorageStore Store at -20
Background

 Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).


 


1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.

2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.

3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.

4. Cheung, P. et al. (2000) Cell 103, 263-71.

5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.

6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.

7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.

8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.

9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.

10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.

11. Dai, J. et al. (2005) Genes Dev 19, 472-88.

Host Species Rabbit

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