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MboII

MboII

  • 商品品牌:AbSci
  • 商品货号:C600582
  • 货期: 预计到货1-5
货号 规格 价格
C600582-0100 100 U ¥0 元
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MboII

Unit definition: One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Recognition sites: GAAGA(N)8↑
Source: E.coli strain, that carries the cloned gene Mbo II from Moraxella bovis
Assayed on: Lambda DNA (dam-)
Optimal buffer: Y?(33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature: 37°C
Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations: After 5-fold overdigestion with enzyme more than 60% of the DNA fragments can be ligated and recut. In presence of 10%PEG ligation is better.
Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methlation effect: Blocked by overlappin dam-methylation(GmATC):?GAAGATC.
Thermal inactivation: enzyme is inactivated by incubation at 65°C for 20 mins.
冷冻(-20℃)

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