您好!欢迎来到Lifesci mall生命科学商城!

客服电话

400-9961-933

当前位置: 首页 > ELISA试剂盒 > ELISA试剂盒 > Human TNF-α ELISA kit

最近浏览过的商品

Human TNF-α ELISA kit

Human TNF-α ELISA kit

  • 商品品牌:AbSci
  • 商品货号:EK0459
  • 货期: 预计到货5-10
货号 规格 价格
EK0459-1 1x96T ¥2394 元
EK0459-2 5x96T ¥10794 元
EK0459-3 10x96T ¥20754 元
购买数量:
  • 商品介绍
  • 商品描述
  • 商品相册
ELISA Kit
applications Solid Phase Sandwich ELISA
Application Details Detect Range: 15.625-1000pg/ml
Sensitivity: 7pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric
Species Reactivity Human
Specificity Natural and recombinant Human TNF-α Ligand
Cross Reactivity No significant interference observed with available related molecules.
Target Name Human TNF-α
Background

The prototype ligand of the TNF superfamily, TNF-α/TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis (1-4). Human cells known to express TNF-α include B cells, colonic columnar epithelial cells, NK and CD3+CD56+ hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4+ and CD8+ T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.

It is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids (aa) in length (4, 5). It contains a 30 aa cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region (6, 7). TNF-α is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNF-α (sTNF-α is released from the C-terminus of the transmembrane protein through the activity of TNF-α-converting enzyme (TACE), a membrane-bound disintegrin metalloproteinase (8, 9).

TNF-α is reported to promote inflammatory cell infiltration by upregulating leukocyte adhesion molecules on endothelial cells, serve as a chemotactic agent for monocytes, and activate phagocyte killing mechanisms (10). Deficiencies in either TNF-α or its receptors can increase susceptibility to infection by intracellular pathogens (11 - 12). TNF- may also play a role in lymphoid tissue development. Knockout mice lack splenic B cell follicles and the ability to form germinal centers (13, 14). Other potential physiological roles for TNF-α and its receptors include regulating the differentiation of hematopoietic stem and progenitor cells (15 - 17).

TNF-α has been implicated in a number of pathophysiological processes. It is associated with unregulated pro-inflammatory activity and is thought to be a critical mediator of endotoxin-induced septic shock (18). Cachexia (or whole body wasting) has also been associated with long-term circulating TNF-α. Other disorders with potential TNF-α involvement include asthma), type 2 diabetes, Crohn

Background References

Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.

Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184.

Sedgwick, J.D. et al. (2000) Immunol. Today 21:110.

MacEwan, D.J. (2002) Brit. J. Pharmacol. 135:855.

Pennica, D. et al. (1984) Nature 312:724.

Wang, A.M. et al. (1985) Science 228:149.

Ishisaka, R. et al. (1999) J. Biochem. 126:413.

Kriegler, M. et al. (1988) Cell 53:45.

Moss, M.L. et al. (1997) Nature 385:733.

Wang, C.Y. et al. (1998) Science 281:1680.

Peschon, J.J. et al. (1998) J. Immunol. 160:943.

Wellmer, A. et al. (2001) Infect. Immun. 69:6881.

Trevejo, J.M. et al. (2001) Proc. Natl. Acad. Sci. USA 98:12162.

Kasahara, S. et al. (2003) J. Virol. 77:2469.

Pasparakis, M. et al. (1996) J. Exp. Med. 184:1397.

Taniguchi, T. et al. (1997) Lab. Invest. 77:647.

Jacobsen, F.W. et al. (1994) Proc. Natl. Acad. Sci. USA 91:10695.

Zhang, Y. et al. (1995) Blood 86:2930.

applications Solid Phase Sandwich ELISA
Application Details Detect Range: 15.625-1000pg/ml
Sensitivity: 7pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric
Species Reactivity Human
Specificity Natural and recombinant Human TNF-α Ligand
Cross Reactivity No significant interference observed with available related molecules.
Target Name Human TNF-α
Background

The prototype ligand of the TNF superfamily, TNF-α/TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis (1-4). Human cells known to express TNF-α include B cells, colonic columnar epithelial cells, NK and CD3+CD56+ hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4+ and CD8+ T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.

It is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids (aa) in length (4, 5). It contains a 30 aa cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region (6, 7). TNF-α is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNF-α (sTNF-α is released from the C-terminus of the transmembrane protein through the activity of TNF-α-converting enzyme (TACE), a membrane-bound disintegrin metalloproteinase (8, 9).

TNF-α is reported to promote inflammatory cell infiltration by upregulating leukocyte adhesion molecules on endothelial cells, serve as a chemotactic agent for monocytes, and activate phagocyte killing mechanisms (10). Deficiencies in either TNF-α or its receptors can increase susceptibility to infection by intracellular pathogens (11 - 12). TNF- may also play a role in lymphoid tissue development. Knockout mice lack splenic B cell follicles and the ability to form germinal centers (13, 14). Other potential physiological roles for TNF-α and its receptors include regulating the differentiation of hematopoietic stem and progenitor cells (15 - 17).

TNF-α has been implicated in a number of pathophysiological processes. It is associated with unregulated pro-inflammatory activity and is thought to be a critical mediator of endotoxin-induced septic shock (18). Cachexia (or whole body wasting) has also been associated with long-term circulating TNF-α. Other disorders with potential TNF-α involvement include asthma), type 2 diabetes, Crohn

Background References

Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.

Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184.

Sedgwick, J.D. et al. (2000) Immunol. Today 21:110.

MacEwan, D.J. (2002) Brit. J. Pharmacol. 135:855.

Pennica, D. et al. (1984) Nature 312:724.

Wang, A.M. et al. (1985) Science 228:149.

Ishisaka, R. et al. (1999) J. Biochem. 126:413.

Kriegler, M. et al. (1988) Cell 53:45.

Moss, M.L. et al. (1997) Nature 385:733.

Wang, C.Y. et al. (1998) Science 281:1680.

Peschon, J.J. et al. (1998) J. Immunol. 160:943.

Wellmer, A. et al. (2001) Infect. Immun. 69:6881.

Trevejo, J.M. et al. (2001) Proc. Natl. Acad. Sci. USA 98:12162.

Kasahara, S. et al. (2003) J. Virol. 77:2469.

Pasparakis, M. et al. (1996) J. Exp. Med. 184:1397.

Taniguchi, T. et al. (1997) Lab. Invest. 77:647.

Jacobsen, F.W. et al. (1994) Proc. Natl. Acad. Sci. USA 91:10695.

Zhang, Y. et al. (1995) Blood 86:2930.

用户评论

(共0条评论)
  • 暂时还没有任何用户评论
总计 0 个记录,共 1 页。 第一页 上一页 下一页 最末页
用户名: 匿名用户
E-mail:
评价等级:
评论内容:
验证码: captcha
版权所有 2005-2017 南京川博生物技术有限公司(Lifesci mall生命科学商城) All rights reserved
公司地址:江苏省南京市浦口国家高新区星火路10号 电话: 400-9961-933 | E-mail: info@lifescimall.com
增值电信业务经营许可证: 苏B1-20150380 苏ICP备15009006号-2
本商城logo和图片都已申请保护,未经授权不得使用。本商城承诺不会将顾客信息泄漏给任何第三方机构和个人。
有任何购物问题请联系我们在线客服。
  • 电话咨询

  • 400-9961-933