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GFP-Tag antibody

GFP-Tag antibody

  • 商品品牌:AbSci
  • 商品货号:ABT529
  • 货期: 预计到货1-5
货号 规格 价格
ABT529-1 50ul ¥800 元
ABT529-2 100ul ¥1200 元
购买数量:
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Primary Antibody
Clonality Polyclonal
Purification Antibodies were purified by affinity purification using immunogen.
applications WB
Application Details Western blotting: 1:2000 - 1:3000
Specificity The antibody detects endogenous level of total GFP-Tag antibody.
Target Name GFP-Tag
Immunogen Type Recombinant Protein
Immunogen Description Recombinant protein of GFP.
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore Store at -20˚C
Background Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.
Host Species Rabbit

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